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recombinant mouse cd200 peptide r d systems  (R&D Systems)


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    R&D Systems recombinant mouse cd200 peptide r d systems
    Recombinant Mouse Cd200 Peptide R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+cd200+peptide+r+d+systems/Recombinant+Human+CD200+Fc+Chimera+Protein%2C+CF/pmc09621138__jci___132___150750___s008-9-7-11
    Average 94 stars, based on 7 article reviews
    recombinant mouse cd200 peptide r d systems - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis
    Article Snippet: Vector Laboratories BP-9200-50 Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11055; RRID: AB_2534102 Donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat#A-10042; RRID: AB_2534017 Donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 4 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab97051; RRID: AB_10679369 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab98693; RRID: AB_10674928 Goat Anti-Mouse IgG2a heavy chain (HRP) Abcam Cat#ab97245; RRID: AB_10680049 Rabbit Anti-Goat IgG H&L (HRP) Abcam Cat#ab97100; RRID: AB_10687752 .. Recombinant Human CD200 Peptide R&D Systems Cat#2724-CD-050 Recombinant Mouse CD200 Peptide R&D Systems Cat3355-CD-050 Purified Mouse Anti-Human CD200 BD Biosciences Cat#552023 Recombinant Human MMP3 protein R&D Systems Cat#513-MP-010 Recombinant Human MMP11 (Catalytic domain) Enzo Life Sciences Cat#BML-SE282-0010 Recombinant Human TIMP3 Protein R&D Systems Cat#973-TM Bovine Pituitary Extract ThermoFisher Scientific Cat#13028014 Recombinant Human Fibroblast Growth Factor PeproTech Ltd Cat#AF-100-18B Recombinant Human Epidermal Growth Factor PeproTech Ltd Cat#AF-100-15 5 Phorbol 12-myristate 13- acetate (PMA) Abcam Cat#ab120297 Ionomycin Ca2+ Salt, Ca2+ ionophore Abcam Cat#ab120116 Z-VAD-FMK (Pancaspase inhibitor) Selleckchem Cat#S8102 Z-IETD-FMK (Caspase 8 inhibitor) Selleckchem Cat#S7314 Z-LEHD-FMK (Caspase 9 inhibitor) Selleckchem Cat#S7313 ZB4 clone anti-Fas mAb Merck Millipore Cat#05-338 GW9662 (PPARγ antagonist) Selleckchem Cat#S2915 Dispase Worthington Cat#LS02104 Collagenase III Worthington Cat#LS004182 Hyaluronidase Worthington Cat#LS005475 DNase I Stem Cell Technologies Cat#07900 Donkey Serum Sigma-Aldrich Cat#D9663 VECTASHIELD® Antifade Mounting Medium Vector Laboratories Cat#H-1000-10 Protease Inhibitor Cocktail (100x) Cell Signalling Cat#5871S QuantiTect Reverse Transcription Kit Qiagen Cat#205313 TaqMan® Universal Master Mix II ThermoFisher Scientific Cat#4440040 Bovine Serum Albumin Powder Fisher Bioreagents Cat#BP9702-100 IncuCyte® Caspase-3/7 Reagent for Apoptosis Essen Bioscience Cat#4704 TWEEN®20 Sigma-Aldrich Cat#P9416 IGEPAL®CA-630 Sigma-Aldrich Cat#I8896 6 Immobilon Forte Western HRP substrate Merck Millipore Cat#WBLUF0100 IlluminaTM TotalPrepTM RNA Amplification Kit ThermoFisher Scientific Cat#AMIL1791 Illumina HumanHT-12 v4 Expression BeadChip Illumina Cat#BD-901-1001 RPMI 1640 Medium ThermoFisher Scientific Cat#11875093 DMEM Medium ThermoFisher Scientific Cat#11960085 Keratinocyte Serum Free Media ThermoFisher Scientific Cat#17005059 Fetal Bovine Serum ThermoFisher Scientific Cat#10270106 Horse Serum, Heat Inactivated ThermoFisher Scientific Cat#26050088 Ficoll® Paque Plus GE Healthcare Cat#GE17-1440-02 Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit, for 40 blots Bio-Rad Cat#1704272 TGXTM FastCastTM Acrylamide Kit, 7.5% Bio-Rad Cat#1610171 96 well plates, flat bottomed, white walled Greiner Bio-One Cat#655098 PierceTM Protein Concentrator PES, 3K MWCO ThermoFisher Scientific Cat#88526 PierceTM BCA Protein Assay Kit ThermoFisher Scientific Cat#23225 RetroNectin® Recombinant Human Fibronectin Fragment TaKaRa Bio Cat#T100A .. CD200 Gift of Alex Tonks, Department of IMAGE consortium; Clone ID 5299899 7 Haematology, Cardiff University, UK Retroviral Expression Vector PINCO Gift of Alex Tonks, Department of Haematology, Cardiff University, UK

    Purification:

    Article Title: CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis
    Article Snippet: Vector Laboratories BP-9200-50 Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11055; RRID: AB_2534102 Donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat#A-10042; RRID: AB_2534017 Donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 4 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab97051; RRID: AB_10679369 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab98693; RRID: AB_10674928 Goat Anti-Mouse IgG2a heavy chain (HRP) Abcam Cat#ab97245; RRID: AB_10680049 Rabbit Anti-Goat IgG H&L (HRP) Abcam Cat#ab97100; RRID: AB_10687752 .. Recombinant Human CD200 Peptide R&D Systems Cat#2724-CD-050 Recombinant Mouse CD200 Peptide R&D Systems Cat3355-CD-050 Purified Mouse Anti-Human CD200 BD Biosciences Cat#552023 Recombinant Human MMP3 protein R&D Systems Cat#513-MP-010 Recombinant Human MMP11 (Catalytic domain) Enzo Life Sciences Cat#BML-SE282-0010 Recombinant Human TIMP3 Protein R&D Systems Cat#973-TM Bovine Pituitary Extract ThermoFisher Scientific Cat#13028014 Recombinant Human Fibroblast Growth Factor PeproTech Ltd Cat#AF-100-18B Recombinant Human Epidermal Growth Factor PeproTech Ltd Cat#AF-100-15 5 Phorbol 12-myristate 13- acetate (PMA) Abcam Cat#ab120297 Ionomycin Ca2+ Salt, Ca2+ ionophore Abcam Cat#ab120116 Z-VAD-FMK (Pancaspase inhibitor) Selleckchem Cat#S8102 Z-IETD-FMK (Caspase 8 inhibitor) Selleckchem Cat#S7314 Z-LEHD-FMK (Caspase 9 inhibitor) Selleckchem Cat#S7313 ZB4 clone anti-Fas mAb Merck Millipore Cat#05-338 GW9662 (PPARγ antagonist) Selleckchem Cat#S2915 Dispase Worthington Cat#LS02104 Collagenase III Worthington Cat#LS004182 Hyaluronidase Worthington Cat#LS005475 DNase I Stem Cell Technologies Cat#07900 Donkey Serum Sigma-Aldrich Cat#D9663 VECTASHIELD® Antifade Mounting Medium Vector Laboratories Cat#H-1000-10 Protease Inhibitor Cocktail (100x) Cell Signalling Cat#5871S QuantiTect Reverse Transcription Kit Qiagen Cat#205313 TaqMan® Universal Master Mix II ThermoFisher Scientific Cat#4440040 Bovine Serum Albumin Powder Fisher Bioreagents Cat#BP9702-100 IncuCyte® Caspase-3/7 Reagent for Apoptosis Essen Bioscience Cat#4704 TWEEN®20 Sigma-Aldrich Cat#P9416 IGEPAL®CA-630 Sigma-Aldrich Cat#I8896 6 Immobilon Forte Western HRP substrate Merck Millipore Cat#WBLUF0100 IlluminaTM TotalPrepTM RNA Amplification Kit ThermoFisher Scientific Cat#AMIL1791 Illumina HumanHT-12 v4 Expression BeadChip Illumina Cat#BD-901-1001 RPMI 1640 Medium ThermoFisher Scientific Cat#11875093 DMEM Medium ThermoFisher Scientific Cat#11960085 Keratinocyte Serum Free Media ThermoFisher Scientific Cat#17005059 Fetal Bovine Serum ThermoFisher Scientific Cat#10270106 Horse Serum, Heat Inactivated ThermoFisher Scientific Cat#26050088 Ficoll® Paque Plus GE Healthcare Cat#GE17-1440-02 Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit, for 40 blots Bio-Rad Cat#1704272 TGXTM FastCastTM Acrylamide Kit, 7.5% Bio-Rad Cat#1610171 96 well plates, flat bottomed, white walled Greiner Bio-One Cat#655098 PierceTM Protein Concentrator PES, 3K MWCO ThermoFisher Scientific Cat#88526 PierceTM BCA Protein Assay Kit ThermoFisher Scientific Cat#23225 RetroNectin® Recombinant Human Fibronectin Fragment TaKaRa Bio Cat#T100A .. CD200 Gift of Alex Tonks, Department of IMAGE consortium; Clone ID 5299899 7 Haematology, Cardiff University, UK Retroviral Expression Vector PINCO Gift of Alex Tonks, Department of Haematology, Cardiff University, UK

    Protease Inhibitor:

    Article Title: CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis
    Article Snippet: Vector Laboratories BP-9200-50 Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11055; RRID: AB_2534102 Donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat#A-10042; RRID: AB_2534017 Donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 4 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab97051; RRID: AB_10679369 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab98693; RRID: AB_10674928 Goat Anti-Mouse IgG2a heavy chain (HRP) Abcam Cat#ab97245; RRID: AB_10680049 Rabbit Anti-Goat IgG H&L (HRP) Abcam Cat#ab97100; RRID: AB_10687752 .. Recombinant Human CD200 Peptide R&D Systems Cat#2724-CD-050 Recombinant Mouse CD200 Peptide R&D Systems Cat3355-CD-050 Purified Mouse Anti-Human CD200 BD Biosciences Cat#552023 Recombinant Human MMP3 protein R&D Systems Cat#513-MP-010 Recombinant Human MMP11 (Catalytic domain) Enzo Life Sciences Cat#BML-SE282-0010 Recombinant Human TIMP3 Protein R&D Systems Cat#973-TM Bovine Pituitary Extract ThermoFisher Scientific Cat#13028014 Recombinant Human Fibroblast Growth Factor PeproTech Ltd Cat#AF-100-18B Recombinant Human Epidermal Growth Factor PeproTech Ltd Cat#AF-100-15 5 Phorbol 12-myristate 13- acetate (PMA) Abcam Cat#ab120297 Ionomycin Ca2+ Salt, Ca2+ ionophore Abcam Cat#ab120116 Z-VAD-FMK (Pancaspase inhibitor) Selleckchem Cat#S8102 Z-IETD-FMK (Caspase 8 inhibitor) Selleckchem Cat#S7314 Z-LEHD-FMK (Caspase 9 inhibitor) Selleckchem Cat#S7313 ZB4 clone anti-Fas mAb Merck Millipore Cat#05-338 GW9662 (PPARγ antagonist) Selleckchem Cat#S2915 Dispase Worthington Cat#LS02104 Collagenase III Worthington Cat#LS004182 Hyaluronidase Worthington Cat#LS005475 DNase I Stem Cell Technologies Cat#07900 Donkey Serum Sigma-Aldrich Cat#D9663 VECTASHIELD® Antifade Mounting Medium Vector Laboratories Cat#H-1000-10 Protease Inhibitor Cocktail (100x) Cell Signalling Cat#5871S QuantiTect Reverse Transcription Kit Qiagen Cat#205313 TaqMan® Universal Master Mix II ThermoFisher Scientific Cat#4440040 Bovine Serum Albumin Powder Fisher Bioreagents Cat#BP9702-100 IncuCyte® Caspase-3/7 Reagent for Apoptosis Essen Bioscience Cat#4704 TWEEN®20 Sigma-Aldrich Cat#P9416 IGEPAL®CA-630 Sigma-Aldrich Cat#I8896 6 Immobilon Forte Western HRP substrate Merck Millipore Cat#WBLUF0100 IlluminaTM TotalPrepTM RNA Amplification Kit ThermoFisher Scientific Cat#AMIL1791 Illumina HumanHT-12 v4 Expression BeadChip Illumina Cat#BD-901-1001 RPMI 1640 Medium ThermoFisher Scientific Cat#11875093 DMEM Medium ThermoFisher Scientific Cat#11960085 Keratinocyte Serum Free Media ThermoFisher Scientific Cat#17005059 Fetal Bovine Serum ThermoFisher Scientific Cat#10270106 Horse Serum, Heat Inactivated ThermoFisher Scientific Cat#26050088 Ficoll® Paque Plus GE Healthcare Cat#GE17-1440-02 Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit, for 40 blots Bio-Rad Cat#1704272 TGXTM FastCastTM Acrylamide Kit, 7.5% Bio-Rad Cat#1610171 96 well plates, flat bottomed, white walled Greiner Bio-One Cat#655098 PierceTM Protein Concentrator PES, 3K MWCO ThermoFisher Scientific Cat#88526 PierceTM BCA Protein Assay Kit ThermoFisher Scientific Cat#23225 RetroNectin® Recombinant Human Fibronectin Fragment TaKaRa Bio Cat#T100A .. CD200 Gift of Alex Tonks, Department of IMAGE consortium; Clone ID 5299899 7 Haematology, Cardiff University, UK Retroviral Expression Vector PINCO Gift of Alex Tonks, Department of Haematology, Cardiff University, UK

    Reverse Transcription:

    Article Title: CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis
    Article Snippet: Vector Laboratories BP-9200-50 Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11055; RRID: AB_2534102 Donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat#A-10042; RRID: AB_2534017 Donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 4 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab97051; RRID: AB_10679369 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab98693; RRID: AB_10674928 Goat Anti-Mouse IgG2a heavy chain (HRP) Abcam Cat#ab97245; RRID: AB_10680049 Rabbit Anti-Goat IgG H&L (HRP) Abcam Cat#ab97100; RRID: AB_10687752 .. Recombinant Human CD200 Peptide R&D Systems Cat#2724-CD-050 Recombinant Mouse CD200 Peptide R&D Systems Cat3355-CD-050 Purified Mouse Anti-Human CD200 BD Biosciences Cat#552023 Recombinant Human MMP3 protein R&D Systems Cat#513-MP-010 Recombinant Human MMP11 (Catalytic domain) Enzo Life Sciences Cat#BML-SE282-0010 Recombinant Human TIMP3 Protein R&D Systems Cat#973-TM Bovine Pituitary Extract ThermoFisher Scientific Cat#13028014 Recombinant Human Fibroblast Growth Factor PeproTech Ltd Cat#AF-100-18B Recombinant Human Epidermal Growth Factor PeproTech Ltd Cat#AF-100-15 5 Phorbol 12-myristate 13- acetate (PMA) Abcam Cat#ab120297 Ionomycin Ca2+ Salt, Ca2+ ionophore Abcam Cat#ab120116 Z-VAD-FMK (Pancaspase inhibitor) Selleckchem Cat#S8102 Z-IETD-FMK (Caspase 8 inhibitor) Selleckchem Cat#S7314 Z-LEHD-FMK (Caspase 9 inhibitor) Selleckchem Cat#S7313 ZB4 clone anti-Fas mAb Merck Millipore Cat#05-338 GW9662 (PPARγ antagonist) Selleckchem Cat#S2915 Dispase Worthington Cat#LS02104 Collagenase III Worthington Cat#LS004182 Hyaluronidase Worthington Cat#LS005475 DNase I Stem Cell Technologies Cat#07900 Donkey Serum Sigma-Aldrich Cat#D9663 VECTASHIELD® Antifade Mounting Medium Vector Laboratories Cat#H-1000-10 Protease Inhibitor Cocktail (100x) Cell Signalling Cat#5871S QuantiTect Reverse Transcription Kit Qiagen Cat#205313 TaqMan® Universal Master Mix II ThermoFisher Scientific Cat#4440040 Bovine Serum Albumin Powder Fisher Bioreagents Cat#BP9702-100 IncuCyte® Caspase-3/7 Reagent for Apoptosis Essen Bioscience Cat#4704 TWEEN®20 Sigma-Aldrich Cat#P9416 IGEPAL®CA-630 Sigma-Aldrich Cat#I8896 6 Immobilon Forte Western HRP substrate Merck Millipore Cat#WBLUF0100 IlluminaTM TotalPrepTM RNA Amplification Kit ThermoFisher Scientific Cat#AMIL1791 Illumina HumanHT-12 v4 Expression BeadChip Illumina Cat#BD-901-1001 RPMI 1640 Medium ThermoFisher Scientific Cat#11875093 DMEM Medium ThermoFisher Scientific Cat#11960085 Keratinocyte Serum Free Media ThermoFisher Scientific Cat#17005059 Fetal Bovine Serum ThermoFisher Scientific Cat#10270106 Horse Serum, Heat Inactivated ThermoFisher Scientific Cat#26050088 Ficoll® Paque Plus GE Healthcare Cat#GE17-1440-02 Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit, for 40 blots Bio-Rad Cat#1704272 TGXTM FastCastTM Acrylamide Kit, 7.5% Bio-Rad Cat#1610171 96 well plates, flat bottomed, white walled Greiner Bio-One Cat#655098 PierceTM Protein Concentrator PES, 3K MWCO ThermoFisher Scientific Cat#88526 PierceTM BCA Protein Assay Kit ThermoFisher Scientific Cat#23225 RetroNectin® Recombinant Human Fibronectin Fragment TaKaRa Bio Cat#T100A .. CD200 Gift of Alex Tonks, Department of IMAGE consortium; Clone ID 5299899 7 Haematology, Cardiff University, UK Retroviral Expression Vector PINCO Gift of Alex Tonks, Department of Haematology, Cardiff University, UK

    Western Blot:

    Article Title: CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis
    Article Snippet: Vector Laboratories BP-9200-50 Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11055; RRID: AB_2534102 Donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat#A-10042; RRID: AB_2534017 Donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 4 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab97051; RRID: AB_10679369 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab98693; RRID: AB_10674928 Goat Anti-Mouse IgG2a heavy chain (HRP) Abcam Cat#ab97245; RRID: AB_10680049 Rabbit Anti-Goat IgG H&L (HRP) Abcam Cat#ab97100; RRID: AB_10687752 .. Recombinant Human CD200 Peptide R&D Systems Cat#2724-CD-050 Recombinant Mouse CD200 Peptide R&D Systems Cat3355-CD-050 Purified Mouse Anti-Human CD200 BD Biosciences Cat#552023 Recombinant Human MMP3 protein R&D Systems Cat#513-MP-010 Recombinant Human MMP11 (Catalytic domain) Enzo Life Sciences Cat#BML-SE282-0010 Recombinant Human TIMP3 Protein R&D Systems Cat#973-TM Bovine Pituitary Extract ThermoFisher Scientific Cat#13028014 Recombinant Human Fibroblast Growth Factor PeproTech Ltd Cat#AF-100-18B Recombinant Human Epidermal Growth Factor PeproTech Ltd Cat#AF-100-15 5 Phorbol 12-myristate 13- acetate (PMA) Abcam Cat#ab120297 Ionomycin Ca2+ Salt, Ca2+ ionophore Abcam Cat#ab120116 Z-VAD-FMK (Pancaspase inhibitor) Selleckchem Cat#S8102 Z-IETD-FMK (Caspase 8 inhibitor) Selleckchem Cat#S7314 Z-LEHD-FMK (Caspase 9 inhibitor) Selleckchem Cat#S7313 ZB4 clone anti-Fas mAb Merck Millipore Cat#05-338 GW9662 (PPARγ antagonist) Selleckchem Cat#S2915 Dispase Worthington Cat#LS02104 Collagenase III Worthington Cat#LS004182 Hyaluronidase Worthington Cat#LS005475 DNase I Stem Cell Technologies Cat#07900 Donkey Serum Sigma-Aldrich Cat#D9663 VECTASHIELD® Antifade Mounting Medium Vector Laboratories Cat#H-1000-10 Protease Inhibitor Cocktail (100x) Cell Signalling Cat#5871S QuantiTect Reverse Transcription Kit Qiagen Cat#205313 TaqMan® Universal Master Mix II ThermoFisher Scientific Cat#4440040 Bovine Serum Albumin Powder Fisher Bioreagents Cat#BP9702-100 IncuCyte® Caspase-3/7 Reagent for Apoptosis Essen Bioscience Cat#4704 TWEEN®20 Sigma-Aldrich Cat#P9416 IGEPAL®CA-630 Sigma-Aldrich Cat#I8896 6 Immobilon Forte Western HRP substrate Merck Millipore Cat#WBLUF0100 IlluminaTM TotalPrepTM RNA Amplification Kit ThermoFisher Scientific Cat#AMIL1791 Illumina HumanHT-12 v4 Expression BeadChip Illumina Cat#BD-901-1001 RPMI 1640 Medium ThermoFisher Scientific Cat#11875093 DMEM Medium ThermoFisher Scientific Cat#11960085 Keratinocyte Serum Free Media ThermoFisher Scientific Cat#17005059 Fetal Bovine Serum ThermoFisher Scientific Cat#10270106 Horse Serum, Heat Inactivated ThermoFisher Scientific Cat#26050088 Ficoll® Paque Plus GE Healthcare Cat#GE17-1440-02 Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit, for 40 blots Bio-Rad Cat#1704272 TGXTM FastCastTM Acrylamide Kit, 7.5% Bio-Rad Cat#1610171 96 well plates, flat bottomed, white walled Greiner Bio-One Cat#655098 PierceTM Protein Concentrator PES, 3K MWCO ThermoFisher Scientific Cat#88526 PierceTM BCA Protein Assay Kit ThermoFisher Scientific Cat#23225 RetroNectin® Recombinant Human Fibronectin Fragment TaKaRa Bio Cat#T100A .. CD200 Gift of Alex Tonks, Department of IMAGE consortium; Clone ID 5299899 7 Haematology, Cardiff University, UK Retroviral Expression Vector PINCO Gift of Alex Tonks, Department of Haematology, Cardiff University, UK

    RNA Amplification:

    Article Title: CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis
    Article Snippet: Vector Laboratories BP-9200-50 Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11055; RRID: AB_2534102 Donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat#A-10042; RRID: AB_2534017 Donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 4 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab97051; RRID: AB_10679369 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab98693; RRID: AB_10674928 Goat Anti-Mouse IgG2a heavy chain (HRP) Abcam Cat#ab97245; RRID: AB_10680049 Rabbit Anti-Goat IgG H&L (HRP) Abcam Cat#ab97100; RRID: AB_10687752 .. Recombinant Human CD200 Peptide R&D Systems Cat#2724-CD-050 Recombinant Mouse CD200 Peptide R&D Systems Cat3355-CD-050 Purified Mouse Anti-Human CD200 BD Biosciences Cat#552023 Recombinant Human MMP3 protein R&D Systems Cat#513-MP-010 Recombinant Human MMP11 (Catalytic domain) Enzo Life Sciences Cat#BML-SE282-0010 Recombinant Human TIMP3 Protein R&D Systems Cat#973-TM Bovine Pituitary Extract ThermoFisher Scientific Cat#13028014 Recombinant Human Fibroblast Growth Factor PeproTech Ltd Cat#AF-100-18B Recombinant Human Epidermal Growth Factor PeproTech Ltd Cat#AF-100-15 5 Phorbol 12-myristate 13- acetate (PMA) Abcam Cat#ab120297 Ionomycin Ca2+ Salt, Ca2+ ionophore Abcam Cat#ab120116 Z-VAD-FMK (Pancaspase inhibitor) Selleckchem Cat#S8102 Z-IETD-FMK (Caspase 8 inhibitor) Selleckchem Cat#S7314 Z-LEHD-FMK (Caspase 9 inhibitor) Selleckchem Cat#S7313 ZB4 clone anti-Fas mAb Merck Millipore Cat#05-338 GW9662 (PPARγ antagonist) Selleckchem Cat#S2915 Dispase Worthington Cat#LS02104 Collagenase III Worthington Cat#LS004182 Hyaluronidase Worthington Cat#LS005475 DNase I Stem Cell Technologies Cat#07900 Donkey Serum Sigma-Aldrich Cat#D9663 VECTASHIELD® Antifade Mounting Medium Vector Laboratories Cat#H-1000-10 Protease Inhibitor Cocktail (100x) Cell Signalling Cat#5871S QuantiTect Reverse Transcription Kit Qiagen Cat#205313 TaqMan® Universal Master Mix II ThermoFisher Scientific Cat#4440040 Bovine Serum Albumin Powder Fisher Bioreagents Cat#BP9702-100 IncuCyte® Caspase-3/7 Reagent for Apoptosis Essen Bioscience Cat#4704 TWEEN®20 Sigma-Aldrich Cat#P9416 IGEPAL®CA-630 Sigma-Aldrich Cat#I8896 6 Immobilon Forte Western HRP substrate Merck Millipore Cat#WBLUF0100 IlluminaTM TotalPrepTM RNA Amplification Kit ThermoFisher Scientific Cat#AMIL1791 Illumina HumanHT-12 v4 Expression BeadChip Illumina Cat#BD-901-1001 RPMI 1640 Medium ThermoFisher Scientific Cat#11875093 DMEM Medium ThermoFisher Scientific Cat#11960085 Keratinocyte Serum Free Media ThermoFisher Scientific Cat#17005059 Fetal Bovine Serum ThermoFisher Scientific Cat#10270106 Horse Serum, Heat Inactivated ThermoFisher Scientific Cat#26050088 Ficoll® Paque Plus GE Healthcare Cat#GE17-1440-02 Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit, for 40 blots Bio-Rad Cat#1704272 TGXTM FastCastTM Acrylamide Kit, 7.5% Bio-Rad Cat#1610171 96 well plates, flat bottomed, white walled Greiner Bio-One Cat#655098 PierceTM Protein Concentrator PES, 3K MWCO ThermoFisher Scientific Cat#88526 PierceTM BCA Protein Assay Kit ThermoFisher Scientific Cat#23225 RetroNectin® Recombinant Human Fibronectin Fragment TaKaRa Bio Cat#T100A .. CD200 Gift of Alex Tonks, Department of IMAGE consortium; Clone ID 5299899 7 Haematology, Cardiff University, UK Retroviral Expression Vector PINCO Gift of Alex Tonks, Department of Haematology, Cardiff University, UK

    Expressing:

    Article Title: CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis
    Article Snippet: Vector Laboratories BP-9200-50 Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11055; RRID: AB_2534102 Donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat#A-10042; RRID: AB_2534017 Donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 4 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab97051; RRID: AB_10679369 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab98693; RRID: AB_10674928 Goat Anti-Mouse IgG2a heavy chain (HRP) Abcam Cat#ab97245; RRID: AB_10680049 Rabbit Anti-Goat IgG H&L (HRP) Abcam Cat#ab97100; RRID: AB_10687752 .. Recombinant Human CD200 Peptide R&D Systems Cat#2724-CD-050 Recombinant Mouse CD200 Peptide R&D Systems Cat3355-CD-050 Purified Mouse Anti-Human CD200 BD Biosciences Cat#552023 Recombinant Human MMP3 protein R&D Systems Cat#513-MP-010 Recombinant Human MMP11 (Catalytic domain) Enzo Life Sciences Cat#BML-SE282-0010 Recombinant Human TIMP3 Protein R&D Systems Cat#973-TM Bovine Pituitary Extract ThermoFisher Scientific Cat#13028014 Recombinant Human Fibroblast Growth Factor PeproTech Ltd Cat#AF-100-18B Recombinant Human Epidermal Growth Factor PeproTech Ltd Cat#AF-100-15 5 Phorbol 12-myristate 13- acetate (PMA) Abcam Cat#ab120297 Ionomycin Ca2+ Salt, Ca2+ ionophore Abcam Cat#ab120116 Z-VAD-FMK (Pancaspase inhibitor) Selleckchem Cat#S8102 Z-IETD-FMK (Caspase 8 inhibitor) Selleckchem Cat#S7314 Z-LEHD-FMK (Caspase 9 inhibitor) Selleckchem Cat#S7313 ZB4 clone anti-Fas mAb Merck Millipore Cat#05-338 GW9662 (PPARγ antagonist) Selleckchem Cat#S2915 Dispase Worthington Cat#LS02104 Collagenase III Worthington Cat#LS004182 Hyaluronidase Worthington Cat#LS005475 DNase I Stem Cell Technologies Cat#07900 Donkey Serum Sigma-Aldrich Cat#D9663 VECTASHIELD® Antifade Mounting Medium Vector Laboratories Cat#H-1000-10 Protease Inhibitor Cocktail (100x) Cell Signalling Cat#5871S QuantiTect Reverse Transcription Kit Qiagen Cat#205313 TaqMan® Universal Master Mix II ThermoFisher Scientific Cat#4440040 Bovine Serum Albumin Powder Fisher Bioreagents Cat#BP9702-100 IncuCyte® Caspase-3/7 Reagent for Apoptosis Essen Bioscience Cat#4704 TWEEN®20 Sigma-Aldrich Cat#P9416 IGEPAL®CA-630 Sigma-Aldrich Cat#I8896 6 Immobilon Forte Western HRP substrate Merck Millipore Cat#WBLUF0100 IlluminaTM TotalPrepTM RNA Amplification Kit ThermoFisher Scientific Cat#AMIL1791 Illumina HumanHT-12 v4 Expression BeadChip Illumina Cat#BD-901-1001 RPMI 1640 Medium ThermoFisher Scientific Cat#11875093 DMEM Medium ThermoFisher Scientific Cat#11960085 Keratinocyte Serum Free Media ThermoFisher Scientific Cat#17005059 Fetal Bovine Serum ThermoFisher Scientific Cat#10270106 Horse Serum, Heat Inactivated ThermoFisher Scientific Cat#26050088 Ficoll® Paque Plus GE Healthcare Cat#GE17-1440-02 Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit, for 40 blots Bio-Rad Cat#1704272 TGXTM FastCastTM Acrylamide Kit, 7.5% Bio-Rad Cat#1610171 96 well plates, flat bottomed, white walled Greiner Bio-One Cat#655098 PierceTM Protein Concentrator PES, 3K MWCO ThermoFisher Scientific Cat#88526 PierceTM BCA Protein Assay Kit ThermoFisher Scientific Cat#23225 RetroNectin® Recombinant Human Fibronectin Fragment TaKaRa Bio Cat#T100A .. CD200 Gift of Alex Tonks, Department of IMAGE consortium; Clone ID 5299899 7 Haematology, Cardiff University, UK Retroviral Expression Vector PINCO Gift of Alex Tonks, Department of Haematology, Cardiff University, UK

    Bicinchoninic Acid Protein Assay:

    Article Title: CD200 ectodomain shedding into the tumor microenvironment leads to NK cell dysfunction and apoptosis
    Article Snippet: Vector Laboratories BP-9200-50 Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Invitrogen Cat#A-11055; RRID: AB_2534102 Donkey anti-Rabbit IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Invitrogen Cat#A-10042; RRID: AB_2534017 Donkey anti-Mouse IgG (H+L) Highly CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Invitrogen Cat#A-31571; RRID: AB_162542 4 Goat Anti-Rabbit IgG H&L (HRP) Abcam Cat#ab97051; RRID: AB_10679369 Goat Anti-Mouse IgG1 (HRP) Abcam Cat#ab98693; RRID: AB_10674928 Goat Anti-Mouse IgG2a heavy chain (HRP) Abcam Cat#ab97245; RRID: AB_10680049 Rabbit Anti-Goat IgG H&L (HRP) Abcam Cat#ab97100; RRID: AB_10687752 .. Recombinant Human CD200 Peptide R&D Systems Cat#2724-CD-050 Recombinant Mouse CD200 Peptide R&D Systems Cat3355-CD-050 Purified Mouse Anti-Human CD200 BD Biosciences Cat#552023 Recombinant Human MMP3 protein R&D Systems Cat#513-MP-010 Recombinant Human MMP11 (Catalytic domain) Enzo Life Sciences Cat#BML-SE282-0010 Recombinant Human TIMP3 Protein R&D Systems Cat#973-TM Bovine Pituitary Extract ThermoFisher Scientific Cat#13028014 Recombinant Human Fibroblast Growth Factor PeproTech Ltd Cat#AF-100-18B Recombinant Human Epidermal Growth Factor PeproTech Ltd Cat#AF-100-15 5 Phorbol 12-myristate 13- acetate (PMA) Abcam Cat#ab120297 Ionomycin Ca2+ Salt, Ca2+ ionophore Abcam Cat#ab120116 Z-VAD-FMK (Pancaspase inhibitor) Selleckchem Cat#S8102 Z-IETD-FMK (Caspase 8 inhibitor) Selleckchem Cat#S7314 Z-LEHD-FMK (Caspase 9 inhibitor) Selleckchem Cat#S7313 ZB4 clone anti-Fas mAb Merck Millipore Cat#05-338 GW9662 (PPARγ antagonist) Selleckchem Cat#S2915 Dispase Worthington Cat#LS02104 Collagenase III Worthington Cat#LS004182 Hyaluronidase Worthington Cat#LS005475 DNase I Stem Cell Technologies Cat#07900 Donkey Serum Sigma-Aldrich Cat#D9663 VECTASHIELD® Antifade Mounting Medium Vector Laboratories Cat#H-1000-10 Protease Inhibitor Cocktail (100x) Cell Signalling Cat#5871S QuantiTect Reverse Transcription Kit Qiagen Cat#205313 TaqMan® Universal Master Mix II ThermoFisher Scientific Cat#4440040 Bovine Serum Albumin Powder Fisher Bioreagents Cat#BP9702-100 IncuCyte® Caspase-3/7 Reagent for Apoptosis Essen Bioscience Cat#4704 TWEEN®20 Sigma-Aldrich Cat#P9416 IGEPAL®CA-630 Sigma-Aldrich Cat#I8896 6 Immobilon Forte Western HRP substrate Merck Millipore Cat#WBLUF0100 IlluminaTM TotalPrepTM RNA Amplification Kit ThermoFisher Scientific Cat#AMIL1791 Illumina HumanHT-12 v4 Expression BeadChip Illumina Cat#BD-901-1001 RPMI 1640 Medium ThermoFisher Scientific Cat#11875093 DMEM Medium ThermoFisher Scientific Cat#11960085 Keratinocyte Serum Free Media ThermoFisher Scientific Cat#17005059 Fetal Bovine Serum ThermoFisher Scientific Cat#10270106 Horse Serum, Heat Inactivated ThermoFisher Scientific Cat#26050088 Ficoll® Paque Plus GE Healthcare Cat#GE17-1440-02 Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit, for 40 blots Bio-Rad Cat#1704272 TGXTM FastCastTM Acrylamide Kit, 7.5% Bio-Rad Cat#1610171 96 well plates, flat bottomed, white walled Greiner Bio-One Cat#655098 PierceTM Protein Concentrator PES, 3K MWCO ThermoFisher Scientific Cat#88526 PierceTM BCA Protein Assay Kit ThermoFisher Scientific Cat#23225 RetroNectin® Recombinant Human Fibronectin Fragment TaKaRa Bio Cat#T100A .. CD200 Gift of Alex Tonks, Department of IMAGE consortium; Clone ID 5299899 7 Haematology, Cardiff University, UK Retroviral Expression Vector PINCO Gift of Alex Tonks, Department of Haematology, Cardiff University, UK



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